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Otto Zykov
Otto Zykov

Food Analysis Laboratory Manual Second Edition



This second edition laboratory manual was written to accompany Food Analysis, Fourth Edition, ISBN 978-1-4419-1477-4, by the same author. The 21 laboratory exercises in the manual cover 20 of the 32 chapters in the textbook. Many of the laboratory exercises have multiple sections to cover several methods of analysis for a particular food component of characteristic. Most of the laboratory exercises include the following: introduction, reading assignment, objective, principle of method, chemicals, reagents, precautions and waste disposal, supplies, equipment, procedure, data and calculations, questions, and references. This laboratory manual is ideal for the laboratory portion of undergraduate courses in food analysis.




Food Analysis Laboratory Manual Second Edition



This third edition laboratory manual was written to accompany Food Analysis, Fifth Edition, by the same author. New to this third edition of the laboratory manual are four introductory chapters that complement both the textbook chapters and the laboratory exercises. The 24 laboratory exercises in the manual cover 21 of the 35 chapters in the textbook. Many of the laboratory exercises have multiple sections to cover several methods of analysis for a particular food component or characteristic. Most of the laboratory exercises include the following: background, reading assignment, objective, principle of method, chemicals, reagents, precautions and waste disposal, supplies, equipment, procedure, data and calculations, questions, and references. This laboratory manual is ideal for the laboratory portion of undergraduate courses in food analysis.


S. Suzanne Nielsen, a Professor in the Food Science Department at Purdue University, has taught Food Analysis lecture classes for 32 years, and Food Analysis laboratory courses for 19 years. She has received teaching awards from her department, college, university, and the Institute of Food Technologists. She has edited five editions of a textbook and three editions of a laboratory manual for Food Analysis.


FDA's Bacteriological Analytical Manual (BAM) presents the agency's preferred laboratory procedures for microbiological analyses of foods and cosmetics. AOAC International published previous editions of this manual in a loose-leaf notebook format, and, more recently, on CD-ROM. This online BAM is now available to the public. Some changes have been made to methods since the previous version. A listing of chapters updated since the last hard-copy version (Edition 8, Revision A /1998) can be found in About the Bacteriological Analytical Manual. The members of the BAM Council are listed below. In addition recent changes for most Chapters are documented in a brief Revision History at the beginning of the Method. There is also e-mail contact information for each Chapter. Chapter numbers have been retained from the previous version. However, for this Table of Contents, chapters have been grouped by category. Please send comments to Karen Jinneman.


Innovations in methods for the microbiological analysis of food continue to appear at a rapid pace. Edition 8 (1995) of the Bacteriological Analytical Manual (BAM-8) contained numerous refinements of procedures and updates of references from the 1992 edition. The list of commercially available test kits and the discussion of rapid methods in Appendix 1 were thoroughly revised. Three chapters were added: the use of reverse transcription (RT) and the polymerase chain reaction (PCR) to detect and quantify contamination of shellfish with hepatitis A virus (Chapter 26); new procedures for the alkaline phosphatase test to determine whether dairy foods were prepared with pasteurized milk (Chapter 27); and the use of PCR to detect toxigenic Vibrio cholerae in foods (Chapter 28). For this printing (BAM - 8A), the following has been revised or added: Campylobacter (Chapter 7), Yeast and Molds (Chapter 18), Cyclospora [Chapter 19 (Parasites)] and Staphylococcus enterotoxins (Chapter13). In addition, there are updated tables in Appendix 1 on Rapid Methods and revised and corrected tables in Appendix 2 on MPN. Appendix 3 reflects changes in media and corrects errors in the 8th Edition. A table summarizing changes from BAM-8 to BAM-8A is included.


The methods described in Chapters 1 to 28 are those preferred by FDA for the microbiological analysis of foods, drinks, and cosmetics as well as for their containers, contact materials, and the production environment. This is not necessarily the case for the rapid methods listed in Appendix 1: this appendix is a listing of different kits that are commercially available. These methods have not necessarily been evaluated by FDA, and listing of a method in this appendix does not constitute a recommendation.


Analytical Food Microbiology: A Laboratory Manual develops the practical skills and knowledge required by students and trainees to assess the microbiological quality and safety of food. This user-friendly textbook covers laboratory safety, basic microbiological techniques, evaluation of food for various microbiological groups, detection and enumeration of foodborne pathogens, and control of undesirable foodborne microorganisms. Each well-defined experiment includes clear learning objectives and detailed explanations to help learners understand essential techniques and approaches in applied microbiology.


The fully revised second edition presents improved conventional techniques, advanced analytical methodologies, updated content reflecting emerging food safety concerns, and new laboratory experiments incorporating commercially available microbiological media. Throughout the book, clear and concise chapters explain culture- and molecular-based approaches for assessing microbial quality and safety of diverse foods. This expanded and updated resource:


In the more than ten years since the publication of the internationally acclaimed and bestselling first edition, many changes have taken place in the approaches used to solve problems in food preservation, processing, storage, marketing, consumption, and even after consumption.


This section describes different methods of analyzing cells after transfection, such as flow cytometry, fluorometry, laser-scanning molecular imaging, luminometry, real-time qPCR, microscopy, spectrometry, and western blot analysis. It also provides guidelines and methods that you can use for the analysis of transfected cells. The methods can be used to determine transfection efficiencies and to perform more in-depth analyses of the expression of your favorite gene/protein. However, depending on your cells, reagents, or equipment availability these methods should be modified to fit your laboratory needs.


For more information about luminometry and luciferase assays, see Sambrook J and Russell D (2001). Analysis of gene expression in cultured mammalian cells. In Molecular Cloning: A laboratory manual, Third Ed. (Woodbury, NY: Cold Spring Harbor Laboratory Press), 17.96.


The book is divided into 11 parts. The first 6 parts introduce the foundations of microbiology: the development of microbiology, the structure of microorganisms, microbial growth and its control, metabolism, molecular biology and genetics, DNA technology and genomics, and the nature of viruses. Part Seven is a survey of the microbial world. In the fifth edition, the bacterial survey closely follows the general organization of the forthcoming second edition of Bergey's Manual of Systematic Bacteriology. Although principal attention is devoted to bacteria, eucaryotic microorganisms receive more than usual coverage. Fungi, algae, and protozoa are important in their own right. The introduction to their biology in chapters 25-27 is essential to understanding topics as diverse as clinical microbiology and microbial ecology. Part Eight focuses on the relationships of microorganisms to other organisms and the environment (microbial ecology). It also introduces aquatic and terrestrial microbiology. Chapter 28 presents the general principles underlying microbial ecology and environmental microbiology so that the subsequent chapters on aquatic and terrestrial habitats can be used without excessive redundancy. The chapter also describes various types of microbial interactions such as mutualism, protocooperation, commensalism, and predation that occur in the environment. Parts Nine and Ten are concerned with pathogenicity, resistance, and disease. The three chapters in Part Nine describe normal microbiota, nonspecific host resistance, the major aspects of the immune response, and medical immunology. Part Ten first covers such essential topics as microbial pathogenicity, antimicrobial chemotherapy, and epidemiology. Then chapters 38-40 survey the major human microbial diseases. The disease survey is primarily organized taxonomically on the chapter level; within each chapter diseases are covered according to mode of transmission. This approach provides flexibility and allows the student easy access to information concerning any disease of interest. The survey is not a simple catalog of diseases; diseases are included because of their medical importance and their ability to illuminate the basic principles of disease and resistance. Part Eleven concludes the text with an introduction to food and industrial microbiology. Five appendices aid the student with a review of some basic chemical concepts and with extra information about important topics not completely covered in the text.


Chapter 19--Material on polyphasic taxonomy and the effects of horizontal gene transfer on phylogenetic trees has been added. The introduction to the second edition of Bergey's Manual has been revised and updated.


Besides the chapter aids the text also contains a glossary, an index, and five appendices. The extensive glossary defines the most important terms from each chapter and includes page references. Where desirable, phonetic pronunciations also are given. Most of the glossary definitions have not been taken directly from the text but have been rewritten to give the student further understanding of the item. To improve ease of use, the fifth edition has a large, detailed index. It has been carefully designed to make text material more accessible. The appendices aid the student with extra review of chemical principles and metabolic pathways and provide further details about the taxonomy of bacteria and viruses. To aid the student in following the rapidly changing field of procaryotic taxonomy, appendix III provides the classification of procaryotes according to the first edition of Bergey's Manual of Systematic Bacteriology, and appendix IV gives the classification used by the upcoming second edition of Bergey's Manual. 041b061a72


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